Products

Product items

Product name: Pixel illuminator-C

We supply functionality on microscope

Feature 1: Direct connection to camera port of microscope

•No additional attaching tool is necessary
•No user alignment is necessary

Feature 2: Laser manipulation control on GUI

Pros/Cons against DMD mirror method

Principles

Features

Pros / Cons

Methods of improve of Simultaneous illumination

Methods of improve of Simultaneous illumination (2)

Using two beam scanning system (ordermade)
Laser beam illumination profile can be controlled with 1msec resolution.
The beam size enlargement with focus adjustment unit can be applied independently.

Demo Movie 1 CW laser illumination (Selected area illumination )

Boundary selection is set by mouse clicking on image. Laser illumination amount can be adjusted by moving speed and laser intensity

Demo Movie 2 Laser cell selection (Pulsedlaser)

Selected cells are killed by laser illumination in closed chamber. Observation is fluorescent

Demo Movie 3 Object tracking

Laser illumination with image analysis (intensity analysis) is also possible

Product items

Product name: Sterile-cam USB (STC-USB-M01, -C01)

We provide availability of USB3 camera to your experiments in a sterile environment.

Feature 1: versatility of camera function

Feature 2: accept sterilization process

Comparison of Decontamination and Sterilization

•Some virus and bacteria can remain after Decontamination process

Picture of Sterile-cam USB

Specifications

　　Size: 56mm x 56mm x 135mm (excluding protrusions and cables)

　　Weight: 800g

　　Sensor type: 1/2” CMOS (monochrome or color)

　　Number of pixels: 1.3Mpixels ( 1280 x 1024 )

　　Data interface: USB3( cable length: 2m)

　　Lens mount format: C

 •Attaching Software

　　Camera Explorer (Baumer): capture still images and movies

　　Time lapse image capture (Pinpoint Photonics): continuous image with fixed time intervals

          Captured image files can directly opened and converted to movie file by ImageJ.

          Movie examples captured by attaching time lapse software and converted by ImageJ.

Remarks

Sterile-cam USB accepts only H2O2 Sterilization, it does not accept other sterilization process such as EOG and autoclave.

Option

Application hearing software

Image analysis / edge detection software developed by UCLA Prof. Bahram Jalali laboratory

Comparison with previous edge detection software

Feature of PST

Feature 1: textures and features are detected similarly without any influence of brightness

Feature 2: specimens are easily detected however illumination is insufficient

Feasibility test１: stable texture detection against different illumination level

Background:

As a texture value which is used in image based AI analysis and machine learning is based on pixel intensities, the value changes with illumination level. And a morphology value which is also used in AI analysis may change with boundary width.
Test result:

We prepared intentionally made 6 images with different illumination. However the texture values are varied with different illumination level without PST, the texture values are stabled with PST

Feasibility test 2: cell daughter analysis from dark images with 1/4 brightness

Background:

It is hard to make cell daughter analysis with photo toxic cells because cells have damages from illumination.
Test result:

By applying PST on time lapse images whose brightness level is darkened to 1/4 level, cell daughter　analysis of B cells is successively made. Collaborator:  Prof. Alexander Hoffmann, Signaling Systems Laboratory, UCLA

As a collaboration work with Prof. Jalali, Pinpoint Photonics, Inc. is searching business application of PST